Km. Shih et Am. Fallon, Two-dimensional electrophoretic analysis of Aedes aegypti mosquito fat body proteins during a gonotropic cycle, AM J TROP M, 65(1), 2001, pp. 42-46
Citations number
19
Categorie Soggetti
Envirnomentale Medicine & Public Health","Medical Research General Topics
We have used two-dimensional polyacrylamide gel electrophoresis to examine
changes in the relative abundance and diversity of non-secreted proteins in
Aedes aegypti fat body preparations during a reproductive cycle. Electroph
oretic profiles were evaluated at four time points after eclosion, and at f
ive time points after a blood meal. In contrast to the dramatic changes in
abundance of specific secreted proteins such as vitellogenin, our results s
how that the complement of proteins internal to the fat body remains relati
vely constant during the mosquito reproductive cycle. Of the approximately
5-10% of proteins that do change in abundance, the majority undergo a drama
tic decrease within 24 hours after eclosion.