Evaluation of some garlic (Allium sativum L.) mutants resistant to white rot disease by RAPD analysis

Citation
I. Nabulsi et al., Evaluation of some garlic (Allium sativum L.) mutants resistant to white rot disease by RAPD analysis, ANN AP BIOL, 138(2), 2001, pp. 197-202
Citations number
18
Categorie Soggetti
Agriculture/Agronomy
Journal title
ANNALS OF APPLIED BIOLOGY
ISSN journal
00034746 → ACNP
Volume
138
Issue
2
Year of publication
2001
Pages
197 - 202
Database
ISI
SICI code
0003-4746(2001)138:2<197:EOSG(S>2.0.ZU;2-6
Abstract
Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genet ic diversity among eight garlic mutants resistant to white rot disease (Scl erotium cepivorum). Twelve of the 13 synthetic random primers were found to identify polymorphism in amplification products. Mutants characterised wit h moderate resistance to white rot were closely related to the control usin g cluster and correlation analyses. On the other hand, highly resistant mut ants were quite distant from the control with low correlation coefficients. The banding patterns produced by primer OPB-15 (GGAGGGTGTT) with highly re sistant mutants may by used as genetic markers for early selection of resis tant plants.