I. Nabulsi et al., Evaluation of some garlic (Allium sativum L.) mutants resistant to white rot disease by RAPD analysis, ANN AP BIOL, 138(2), 2001, pp. 197-202
Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genet
ic diversity among eight garlic mutants resistant to white rot disease (Scl
erotium cepivorum). Twelve of the 13 synthetic random primers were found to
identify polymorphism in amplification products. Mutants characterised wit
h moderate resistance to white rot were closely related to the control usin
g cluster and correlation analyses. On the other hand, highly resistant mut
ants were quite distant from the control with low correlation coefficients.
The banding patterns produced by primer OPB-15 (GGAGGGTGTT) with highly re
sistant mutants may by used as genetic markers for early selection of resis
tant plants.