Investigation of an outbreak caused by methicillin-resistant Staphylococcus aureus in a cardiovascular surgery unit by ribotyping, randomly amplifed polymorphic DNA and pulsed-field gel electrophoresis
Cp. Fung et al., Investigation of an outbreak caused by methicillin-resistant Staphylococcus aureus in a cardiovascular surgery unit by ribotyping, randomly amplifed polymorphic DNA and pulsed-field gel electrophoresis, APMIS, 109(6), 2001, pp. 474-480
An outbreak caused by rapid spread of methicillin-resistant Staphylococcus
aureus (MRSA) in an intensive care unit for cardiovascular surgery was inve
stigated by phenotypic and genotypic methods. Fourteen isolates were collec
ted during a 2-month period from clinical and environmental specimens in th
e unit recently re-opened after reconstruction. The isolates were tested fo
r antibiotic susceptibility patterns and genotyped by automated ribotyping,
randomly amplified polymorphic DNA-PCR (RAPD) analysis and pulsed-field ge
l electrophoresis (PFGE). Automated ribotyping applying EcoRI digestion pro
ved to be of no value in separating the isolates. In contrast, PFGE grouped
the isolates into four clusters different from the reference strain. These
results fully correlated with the antibiograms. Twelve of the isolates wer
e grouped into two clonally related clusters. RAPD analyses grouped the iso
lates into five clusters. Except for two isolates of one patient, which had
different RAPD patterns, PFGE and RAPD analyses presented very similar res
ults. The results verified the usefulness of PFGE in studies of MRSA epidem
ics. A combination of these two methods reduces the time to identification
of an outbreak and increases the accuracy in detection of intraspecies diff
erences.