Reproducibility of retinal mapping using optical coherence tomography

Objective: To assess the reproducibility of retinal thickness measurement u sing commercially available mapping software of optical coherence tomograph y (OCT). Methods: Six radial scans, 6 mm long and centered on the fixation point, we re performed on 10 eyes of 10 healthy volunteers and 10 eyes of 10 diabetic patients with clinically significant macular edema. Retinal thickness was measured automatically using the mapping software of OCT in the 9 macular E arly Treatment Diabetic Retinopathy Study areas and in a central area 500 m um in diameter. Measurement reproducibility was tested by means of 3 series of scans performed by 2 different observers on 2 different days. Results w ere assessed by their repeatability and intraclass correlation coefficients (ICCs). Results: In healthy subjects, intraobserver, interobserver, and intervisit reproducibility of retinal thickness measurements were excellent, with a re peatability coefficient of less than 7 mum and ICCs of greater than 0.89. I n diabetic patients, the repeatability coefficient was less than 21 pm in a ll areas of the macula except one, with an ICC of greater than 0.98. Relati ve variations in measurements were small in both healthy and diabetic subje cts, with reproducibilities of +/-5% and +/-6%, respectively. Conclusion: Retinal mapping software of OCT allows reproducible measurement of retinal thickness in both healthy subjects and diabetic patients with m acular edema.