This paper examines the principal nutritional factors that influence the co
ntent of protein in caw's milk and offers Possible strategies for ensuring
acceptable levels, suitable for both the liquid market and processing. Rath
er than provide a review of the numerous experimental studies, with the pri
ncipal aim of developing predictive relationships, this paper has considere
d a number of key studies where in many cases it has been possible to offer
some interpretation of the results obtained. The paper concludes that unde
rfeeding of protein is rarely a major problem with respect to feeding dairy
cows and the use of additional protein sources, and especially protected a
mino acids, does not appear to be the most promising approach to improving
milk protein levels. In contrast, attention to the intake of metabolisable
energy, and the form of energy does offer real opportunities for improving
milk protein content. Strategic use of different and better quality forages
appears to be fundamental as well as the nature of the carbohydrate compon
ent of the concentrates. In this respect, high quality starch offers consid
erable benefit with both wheat and maize being highly attractive feed sourc
es. In contrast, while digestible fibre sources will improve metabolisable
energy intake and milk yield, they seem to be less effective with respect t
o improving milk protein content and in this respect, the excessive use of
lupins needs to be questioned. Finally, the paper considers the quantitativ
e metabolism of amino acids by the host animal following absorption and con
cludes that both mammary supply and mammary uptake are important in the syn
thesis of milk protein. Increasing the supply of amino acids without the ab
ility of the mammary gland to increase uptake will however lead to modest a
nd sometimes zero responses to additional protein supply.