A novel photosensitizer, 2-butylamino-2-demethoxy-hypocrellin B (2-BA-2-DMHB) - its photodynamic effects on HeLa cells: efficacy and apoptosis

A novel hypocrellin congener, 2-butylamino-2-demethoxy-hypocrellin B (2-BA- 2-DMHB) was found to be an effective photosensitizer. Compared with its par ent compound hypocrellin B (HB), its absorption bands extended toward longe r wavelength and the extinction coefficients raised to some degree (lambda (max) (nm) (log epsilon): 463 (4.06), 583 (4.09), 621 (4.10) for 2-BA-2-DMH B and 466 (4.06), 548 (3.70), 580 (3.52) for HB). And it also had a much hi gher photopotentiation factor than HB (i.e., more than 250 versus 10 at a d ose of 24 J cm(-2) of red light on HeLa cells). This might be correlated to the higher ability of superoxide anion generation and the higher cellular uptake of 2-BA-2-DMHB. Meanwhile, the examinations of Hoechst 33342-labeled nuclei, DNA fragmentation on agarose gel and flow cytometry showed that 2- BA-2-DMHB induces apoptosis in photosensitized HeLa cells more quickly than HB, which might be correlated to the higher cellular uptake of 2-BA-2-DMHB and the difference of their cellular localization. The study suggested tha t 2-BA-2-DMHB was a well-suited candidate for a new generation of photodyna mic therapy photosensitizer. (C) 2001 Elsevier Science B.V. All rights rese rved.