Ja. Benitez et al., ADHERENCE OF VIBRIO-CHOLERAE TO CULTURED DIFFERENTIATED HUMAN INTESTINAL-CELLS - AN IN-VITRO COLONIZATION MODEL, Infection and immunity, 65(8), 1997, pp. 3474-3477
Choleragenic vibrios adhered to and multiplied on monolayers of the hi
ghly differentiated mucin-secreting cell line HT29-18N2. Their adheren
ce followed first-order kinetics, was dependent on the concentration o
f vibrios, and was partially inhibited by lipopolysaccharide. Comparis
on of genetically modified vibrios showed that flagella, an active tox
R gene, and the virulence cassette were not essential for initial bind
ing. Inactivation of the hemagglutinin/protease increased binding. Thi
s highly differentiated human intestinal cell line provides a versatil
e new approach for studying major events occurring during intestinal c
olonization: adherence, multiplication, and detachment.