Hairy roots of Brugmansia candida that grow without agitation: Biotechnological implications

Citation
Si. Pitta-alvarez et Am. Giulietti, Hairy roots of Brugmansia candida that grow without agitation: Biotechnological implications, BIOTECH PR, 17(4), 2001, pp. 661-663
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY PROGRESS
ISSN journal
87567938 → ACNP
Volume
17
Issue
4
Year of publication
2001
Pages
661 - 663
Database
ISI
SICI code
8756-7938(200107/08)17:4<661:HROBCT>2.0.ZU;2-V
Abstract
Hairy roots of Brugmansia candida that grew without agitation were obtained . Kinetics of growth and production of the tropane alkaloids scopolamine an d hyoscyamine, with and without agitation, were studied. The exponential gr owth rate was higher in the roots that were exposed to shaking (0.13 d(-1)) than in the nonagitated ones (0.09 d(-1)). The specific production and the levels per flask of both alkaloids were enhanced without shaking. The use of these roots in large-scale productions could be economically advantageou s. It remains to be seen if the data obtained in shake flasks can be extrap olated to large-scale bioreactors.