Si. Pitta-alvarez et Am. Giulietti, Hairy roots of Brugmansia candida that grow without agitation: Biotechnological implications, BIOTECH PR, 17(4), 2001, pp. 661-663
Hairy roots of Brugmansia candida that grew without agitation were obtained
. Kinetics of growth and production of the tropane alkaloids scopolamine an
d hyoscyamine, with and without agitation, were studied. The exponential gr
owth rate was higher in the roots that were exposed to shaking (0.13 d(-1))
than in the nonagitated ones (0.09 d(-1)). The specific production and the
levels per flask of both alkaloids were enhanced without shaking. The use
of these roots in large-scale productions could be economically advantageou
s. It remains to be seen if the data obtained in shake flasks can be extrap
olated to large-scale bioreactors.