D. Magnin et al., Lipase immobilization into porous chitoxan beads: Activities in aqueous and organic media and lipase localization, BIOTECH PR, 17(4), 2001, pp. 734-737
Lipases were noncovalently immobilized in Chitoxan, a polyionic hydrogel ob
tained by complexation between chitosan and xanthan. The properties of free
and immobilized lipases have been compared. In the aqueous medium, the act
ivity was twice as high for immobilized lipases as for free lipases. Immobi
lized lipases in chitoxan were able to hydrolyze triacylglycerols in three
distinct organic solvent media. At the microstructural level, lipases were
not distributed uniformly in the chitoxan beads. Higher concentrations of l
ipase were found in the outer membrane-like layer of the beads, as compared
with lower concentrations in the inner part of the beads.