Glycoprotein V (GPV) is a subunit of the platelet GPlb-V-IX receptor for vo
n Willebrand factor and thrombin. GPV is cleaved from the platelet surface
during activation by thrombin, but its role in hemostasis is still unknown.
It is reported that GPV knockout mice had a decreased tendency to form art
erial occluding thrombi in an intravital thrombosis model and abnormal plat
elet interaction with the subendothellum. In vitro, GPV-deficient platelets
exhibited defective adhesion to a collagen type I-coated surface under flo
w or static conditions. Aggregation studies demonstrated a decreased respon
se of the GPV-deficient platelets to collagen, reflected by an increased la
g phase and reduced amplitude of aggregation. Responses to adenosine diphos
phate, arachidonic acid, and the thromboxane analog U46619 were normal but
were enhanced to low thrombin concentrations. The defect of GPV null platel
ets made them more sensitive to inhibition by the anti-GPVI monoclonal anti
body (mAb) JAQ1, and this was also the case in aspirin- or apyrase-treated
platelets. Moreover, an mAb (V.3) against the extracellular domain of human
GPV selectively inhibited collagen-induced aggregation in human or rat pla
telets. V.3 injected in rats as a bolus decreased the ex vivo collagen aggr
egation response without affecting the platelet count. Finally, surface pla
smon resonance studies demonstrated binding of recombinant soluble GPV on a
collagen-coupled matrix. In conclusion, GPV binds to collagen and appears
to be required for normal platelet responses to this agonist. (C) 2001 by T
he American Society of Hematology.