Variable protection of beta 3-integrin-deficient mice from thrombosis initiated by different mechanisms

Platelet integrin alpha IIb beta3 (GPII/IIIa) plays a central role in the i nitiation of arterial thrombosis, but its contribution to disseminated micr ovascular thrombosis is less well defined. Therefore, wild-type mice (beta3 (+/+)), beta3-integrin-deficient mice (beta3(-/-)), and wild-type mice trea ted with a hamster monoclonal antibody (1B5) that blocks murine alpha IIb b eta3 function were tested in models of large-vessel and microvascular throm bosis. In the large-vessel model, ferric chloride was used to injure the ca rotid artery, and the time to thrombosis was measured. In beta3(+/+) mice, the median time to occlusion was 6.7 minutes, whereas occlusion did not occ ur in any of the beta3(-/-) mice tested (P < .001). Fab and F(ab ')(2) frag ments of 1B5 increased the median time to occlusion. To initiate systemic i ntravascular thrombosis, prothrombotic agents were administered intravenous ly, and platelet thrombus formation was monitored by the decrease in circul ating platelet count. Three minutes after the injection of adenosine diphos phate (ADP), collagen + epinephrine, or tissue factor, the platelet counts in beta3(+/+) mice decreased by 289, 424, and 429 X 10(3)/muL, respectively . beta3(-/-) mice and wild-type mice pretreated with 1B5 Fab (1 mg/kg, IP) were nearly completely protected from the effects of ADR In contrast, beta3 (-/-) mice were only partially protected from the effects of collagen + epi nephrine and minimally protected from the effects of tissue factor. In all cases, less fibrin became deposited in the lungs of beta3(-/-) mice than in wild-type mice. These results suggest that though alpha IIb beta3 plays a dominant role in large-vessel thrombosis, it plays a variable role in syste mic intravascular thrombosis. (C) 2001 by The American Society of Hematolog y.