Dk. Watt et al., COMPARISON OF OVINE, BOVINE AND PORCINE MUCOSAL HEPARINS AND LOW-MOLECULAR-WEIGHT HEPARINS BY DISACCHARIDE ANALYSES AND C-13 NMR, Carbohydrate polymers, 33(1), 1997, pp. 5-11
Ovine, porcine and bovine heparin were compared by NMR, disaccharide c
omposition, optical rotation, elemental analyses, HPSEC and USP analys
es. There were some differences found in the NMR and disaccharide comp
osition of the heparins. Ovine heparin contained less monosulphated an
d disulphated disaccharides than porcine or bovine mucosal heparins. T
he heparins were approximately equivalent in USP activity. Low molecul
ar weight ovine, porcine and bovine heparin were prepared by both nitr
ous acid and copper/peroxide depolymerisation. Products were compared
to each other and to Fragmin(R) by disaccharide composition, optical r
otation, HPSEC, NMR, elemental analyses, and the USP assay for anticoa
gulant activity. The low molecular weight products showed a greater si
milarity to each other than to the parent heparins and it was conclude
d that the species of origin of a low molecular weight heparin would b
e difficult to determine by these methods alone. (C) 1997 Elsevier Sci
ence Ltd. All rights reserved.