Spatial and temporal dynamics of two alternatively spliced regulatory factors, lens epithelium-derived growth factor (ledgf/p75) and p52, in the nucleus
Y. Nishizawa et al., Spatial and temporal dynamics of two alternatively spliced regulatory factors, lens epithelium-derived growth factor (ledgf/p75) and p52, in the nucleus, CELL TIS RE, 305(1), 2001, pp. 107-114
Regulatory factors, lens epithelium-derived growth factor (LEDGF)/p75 and p
52, are generated from a single LEDGF gene by alternative splicing. They ha
ve identical amino acid residues between positions 1-325, but 205 and 8 of
the remaining residues are different in LEDGF and p52, respectively. LEDGF
promotes growth and survival of many cell types. It has an antiapoptotic fu
nction and is a weak general transcriptional co-activator tor. p52 is a tra
nscriptional activator and an essential splicing factor. We investigated th
e spatial and temporal dynamics of LEDGF/p75 and p52, each being tagged wit
h a fluorescent protein, during the cell cycles of CHO-KI, MCDK, and NRK ce
lls in culture. Both LEDGF/p75 and p52 were localized predominantly in the
nucleus. LEDGF/p75 was distributed diffusely in the nucleoplasm in the G1-p
hase and attached to chromatin heterogeneously during the G2 and M-phases o
f cells. In contrast, p52 was localized in the nuclear periphery during the
G1-phase and formed a speckle pattern at the S-phase. It formed a cylindri
cal pattern around the chromosomes during the M-phases of cells. LEDGF and
p52 on sister chromatids migrated into daughter cells. Thus, LEDGF/p75 and
p52 are localized in distinct nuclear compartments where they can activate
transcription or splicing of pre-mRNAs.