Genetic analysis of "all-fish" growth hormone gene transferred carp (Cyprinus carpio L.) and its F-1 generation

Recombinant "all-fish" growth hormone gene (GH) was microinjected Into the fertilized eggs of carp. A comparison between the growth traits of transgen ics and non-transgenics was carried out, and the transgenic individuals wit h significant "fast-growing" effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and thei r non-transgenic siblings, and showed that the reproductive capacity of tra nsgenics was substantially equivalent to those of the non-transgenics. On t he other hand, the genetic separation and the characteristic distribution o f the F-1 generation were genetically analyzed, which gave solid evidence f or the hypothesis that 2-3 chromosomes are integrated with transgene. In ad dition, the distinct biological effects for multisite-integrated transgenes were further discussed. The present study opens a door for the breeding of "fast-growing" transgenic fish.