Mm. Tarpey et I. Fridovich, Methods of detection of vascular reactive species - Nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite, CIRCUL RES, 89(3), 2001, pp. 224-236
The evanescent nature of reactive oxygen and nitrogen species, the multiple
cellular mechanisms evolved to maintain these substances at low (submicrom
olar) concentrations within the vascular system, and the often multifaceted
nature of their reactivities have made measurement of these compounds with
in the vasculature problematic. This review attempts to provide a critical
description of some of the most common approaches to quantification of nitr
ic oxide, superoxide, hydrogen peroxide, and peroxynitrite, with attention
to key issues that may influence the utility of a particular assay when ada
pted for use in vascular cells and tissues.