AKT-1,-2, and-3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon

Citation
Mj. Zinda et al., AKT-1,-2, and-3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon, CLIN CANC R, 7(8), 2001, pp. 2475-2479
Citations number
12
Categorie Soggetti
Oncology
Journal title
CLINICAL CANCER RESEARCH
ISSN journal
10780432 → ACNP
Volume
7
Issue
8
Year of publication
2001
Pages
2475 - 2479
Database
ISI
SICI code
1078-0432(200108)7:8<2475:AAAEIB>2.0.ZU;2-K
Abstract
Purpose: The AKT/PKB kinase controls many of the intracellular processes th at are dysregulated. in human cancer, including the suppression of apoptosi s and anoilkis and the induction of cell cycle progression. Three isoforms of AKT have been identified: AKT-1, -2, and -3. Selective up-regulation of AKT-3 RNA expression has been reported in hormone-independent breast and pr ostate cancer cell lines suggesting that AKT-3 expression may be increased with breast or prostate tumor progression. To determine whether AKT-3 RNA e xpression is selectively up-regulated in human cancers and whether the patt erns of AKT RNA expression may change with tumor development, we examined A KT isoform expression by RT-PCR in human cancer cell lines, primary human c ancers, and normal human tissues. Experimental Design: AKT-1, -2, and -3 RNA expression was examined by RT-PC R. Because up-regulated AKT-3 expression has been implicated in human breas t and prostate cancer progression, we also examined AKT-3 expression levels by semiquantitative RT-PCR using matched normal/tumor first-strand cDNA pa irs from colon, breast, prostate, and lung cancers. Results: Our data reveal that the overwhelming majority of both normal and tumor tissues express all three of the AKT isoforms. Moreover, semiquantita tive RT-PCR of matched normal/tumor pairs confirmed similar AKT-3 RNA expre ssion levels in both normal and tumor tissue. Conclusions: Our data show that both normal and tumor tissues express all t hree of the AKT isoforms and indicate that tumorigenesis does not involve a dramatic shift in the RNA expression patterns of the three AKT isoforms.