J. Esquena et C. Solans, Influence of the HLB parameter of nonionic surfactants on normal and reversed-phase thin layer chromatography, COLL SURF A, 189(1-3), 2001, pp. 85-92
Citations number
21
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Normal and reverse Thin Layer Chromatography was performed in order to stud
y the relationship between the hydrophilic-lipophilic balance of nonionic s
urfactants and its chromatographic migration. Experiments in both normal (s
ilica-gel) and reverse (octadecyl silane modified) Thin Layer Chromatograph
y plates (TLC) were carried out using the same solvent compositions. The in
fluence of solvent composition in a mixed system has also been studied in o
rder to determine the mechanism of the chromatographic separation of nonion
ic surfactants. It was observed that the chromatographic separation followe
d the model of differential partition of surfactants between liquid pseudop
hases. Chromatographic retention was found to be highly dependent on the nu
mber of ethylene oxide units in the surfactant molecules. In reverse TLC it
was also dependent on the alkyl chain length. The relative capacity factor
(normal respect to reverse) was also found to be highly dependent on the n
umber of ethylene oxide units. (C) 2001 Elsevier Science B.V. All rights re
served.