Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis

Authors
Duetz, WA van Beilen, JB Witholt, B
Citation
Wa. Duetz et al., Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis, CURR OPIN B, 12(4), 2001, pp. 419-425
Citations number
70
Categorie Soggetti
Microbiology
Journal title
CURRENT OPINION IN BIOTECHNOLOGY
ISSN journal
09581669 → ACNP
Volume
12
Issue
4
Year of publication
2001
Pages
419 - 425
Database
ISI
SICI code
0958-1669(200108)12:4<419:UPITNE>2.0.ZU;2-E
Abstract
The unique catalytic properties of oxygenases (the regio-specific and/or en antio-specific hydroxylation of non-activated carbons) are of undisputed bi osynthetic value. Factors that govern the economics of their industrial use include a low k(cat), a frequently decreased k(cat) in recombinant strains , limiting oxygen transfer rates in bioreactors, product inhibition, and th e demanding discovery (screening) process.