Presence of stable active oxygen scavenging enzymes superoxide dismutase, ascorbate peroxidase and catalase in a desiccation-tolerant cyanobacterium Lyngbya arboricola under dry state
Sn. Tripathi et P. Srivastava, Presence of stable active oxygen scavenging enzymes superoxide dismutase, ascorbate peroxidase and catalase in a desiccation-tolerant cyanobacterium Lyngbya arboricola under dry state, CURRENT SCI, 81(2), 2001, pp. 197-200
Freshly collected and one- and two-year-old dry mats of a desiccation-toler
ant cyanobacterium Lyngbya arboricola, inhabiting the bark-surface of Mangi
fera indica exhibited enhancement in the activity of active oxygen scavengi
ng enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catal
ase in their cell-free extracts on increasing the incubation period. Compar
atively, the dry mats showed considerable activity of all these enzymes in
the cell-free extracts, but lower than that of freshly collected mats. Five
, two and three isoforms of SOD, APX and catalase enzymes respectively, wer
e observed in the crude extracts of the freshly collected mats on native PA
GE. SOD comprised of one Mn, one Fe-Mn and three Fe-containing isoforms. Ex
cepting the disappearance of Mn and Fe-Mn SODs and a minor loss in the inte
nsity of the isoforms of APX and catalase on native gels, the AOS enzymes w
ere found stable on storage of the mats for one and two years respectively,
under dry state.