Presence of stable active oxygen scavenging enzymes superoxide dismutase, ascorbate peroxidase and catalase in a desiccation-tolerant cyanobacterium Lyngbya arboricola under dry state

Citation
Sn. Tripathi et P. Srivastava, Presence of stable active oxygen scavenging enzymes superoxide dismutase, ascorbate peroxidase and catalase in a desiccation-tolerant cyanobacterium Lyngbya arboricola under dry state, CURRENT SCI, 81(2), 2001, pp. 197-200
Citations number
24
Categorie Soggetti
Multidisciplinary,Multidisciplinary
Journal title
CURRENT SCIENCE
ISSN journal
00113891 → ACNP
Volume
81
Issue
2
Year of publication
2001
Pages
197 - 200
Database
ISI
SICI code
0011-3891(20010725)81:2<197:POSAOS>2.0.ZU;2-I
Abstract
Freshly collected and one- and two-year-old dry mats of a desiccation-toler ant cyanobacterium Lyngbya arboricola, inhabiting the bark-surface of Mangi fera indica exhibited enhancement in the activity of active oxygen scavengi ng enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catal ase in their cell-free extracts on increasing the incubation period. Compar atively, the dry mats showed considerable activity of all these enzymes in the cell-free extracts, but lower than that of freshly collected mats. Five , two and three isoforms of SOD, APX and catalase enzymes respectively, wer e observed in the crude extracts of the freshly collected mats on native PA GE. SOD comprised of one Mn, one Fe-Mn and three Fe-containing isoforms. Ex cepting the disappearance of Mn and Fe-Mn SODs and a minor loss in the inte nsity of the isoforms of APX and catalase on native gels, the AOS enzymes w ere found stable on storage of the mats for one and two years respectively, under dry state.