Two receptors (CRH receptor type 1 and CRH receptor type 2) have been ident
ified for the stress-induced neuropeptide, CRH and related peptides, urocor
tin, and urocortin II. We previously found marked down-regulation of cardia
c CRH receptor type 2 expression following administration of bacterial endo
toxin, lipopolysaccharide, a model of systemic immune activation, and infla
mmation. We postulated that inflammatory cytokines may regulate CRH recepto
r type 2. We show that systemic IL-1 alpha administration significantly dow
n-regulates CRH receptor type 2 mRNA in mouse heart. In addition, TNF alpha
treatment also reduces CRH receptor type 2 mRNA expression, although the e
ffect was not as marked as with IL-1a. However, CRH receptor type 2 mRNA ex
pression is not altered in adult mouse ventricular cardiomyocytes stimulate
d in vitro with TNF alpha or IL-1 alpha. Thus, cytokine regulation may be i
ndirect. Exogenous administration of corticosterone in vivo or acute restra
int stress also reduces cardiac CRH receptor type 2 mRNA expression, but li
ke cytokines, in vitro corticosterone treatment does not modulate expressio
n in cardiomyocytes. Interestingly, treatment with urocortin significantly
decreases CRH receptor type 2 mRNA in cultured cardiomyocytes. We speculate
that in vivo, inflammatory mediators such as lipopolysaccharide and/or cyt
okines may increase urocortin, which in turn down-regulates CRH receptor ty
pe 2 expression in the heart. Because CRH and urocortin increase cardiac co
ntractility and coronary blood flow, impaired CRH receptor type 2 function
during systemic inflammation may ultimately diminish the adaptive cardiac r
esponse to adverse conditions.