CHEMOATTRACTANT FACTORS (IP-10, MIP-1-ALPHA, IL-16) MESSENGER-RNA EXPRESSION IN MONONUCLEAR-CELLS FROM DIFFERENT TISSUES DURING ACUTE SIVMAC251 INFECTION OF MACAQUES
A. Cheret et al., CHEMOATTRACTANT FACTORS (IP-10, MIP-1-ALPHA, IL-16) MESSENGER-RNA EXPRESSION IN MONONUCLEAR-CELLS FROM DIFFERENT TISSUES DURING ACUTE SIVMAC251 INFECTION OF MACAQUES, Journal of medical primatology, 26(1-2), 1997, pp. 19-26
We have used semiquantitative RT-PCR to monitor the expression of mRNA
encoding chemoattractant factors IP-10, MIP-1 alpha, and IL-16 in fre
shly isolated peripheral blood mononuclear cells (PBMCs), lymph node m
ononuclear cells (LNMCs), and mononuclear cells obtained after broncho
alveolar lavages (BALMCs) of two cynomolgus macaques inoculated intrav
enously with a pathogenic isolate of simian immunodeficiency virus, SI
Vmac251. Concomitant with the peak of systemic viral replication (two
weeks after experimental inoculation) and proinflammatory cytokine IL-
6 mRNA expression, high levels of MIP-1 alpha and IP-10 mRNA were prod
uced in LNMCs and BALMCs. In BALMCs, in which we have reported a marke
d progressive overexpression of IFN-gamma mRNA coinciding with an incr
ease in the CD8+ lymphocyte percentages, we noticed a progressive over
expression of IL-16 mRNA. Our results suggest the role of chemokines I
P-10, MIP-1 alpha, and IL-16 in the development of inflammatory and im
mune responses during the early stages of lentiviral infection.