D-fructose-binding proteins in bull seminal plasma: Isolation and characterization

The heparin-binding activity of bull seminal plasma proteins was inhibited by D-fructose, D-glucose, inulin and glycogen; D-galactose, dextran and man nan had no effect. While the, ejaculated sperm-heparin interaction was not influenced by the presence of saccharides, the heparin-binding activity of epididymal sperm was inhibited by D-fructose. The results of the binding st udies were confirmed by affinity chromatography on immobilized heparin foll owed by elution with monosaccharides. Proteins adsorbed to a heparin-polyac rylamide column and eluted with D-fructose were analyzed by RP RPLC, SDS el ectrophoresis and by determination of the N-terminal amino-acid sequence. R NAase dimer, PDC-109 and metalloproteinase inhibitor (TIMP-2) were identifi ed.