Linuron cytogenetic activity on human lymphocytes treated in vitro. Evaluation of clastogenic and aneugenic potential using cytokinesis block micronucleus assay in combination with fluorescence in situ hybridization (FISH)

Authors
Papapaulou, P Vlastos, D Stephanou, G Demopoulos, NA
Citation
P. Papapaulou et al., Linuron cytogenetic activity on human lymphocytes treated in vitro. Evaluation of clastogenic and aneugenic potential using cytokinesis block micronucleus assay in combination with fluorescence in situ hybridization (FISH), FR ENV BULL, 10(5), 2001, pp. 431-437
Citations number
48
Categorie Soggetti
Environment/Ecology
Journal title
FRESENIUS ENVIRONMENTAL BULLETIN
ISSN journal
10184619 → ACNP
Volume
10
Issue
5
Year of publication
2001
Pages
431 - 437
Database
ISI
SICI code
1018-4619(200105)10:5<431:LCAOHL>2.0.ZU;2-7
Abstract
Linuron is a substituted urea compound registered for use as an herbicide f or the control of a wide variety of annual and perennial broad-leaved and g rassy weeds. In the present study the effect of Linuron (12.5 - 87.5 mug/ml ) on the micronucleus (MN) induction is studied in human peripheral blood l ymphocyte cultures treated in vitro. The application of fluorescence in sit u hybridization (FISH) on Cytochalasin B (Cyt-B) formed binucleated cells h as shown that the induced micronuclei are due to clastogenic as well as ane ugenic activity.