Mh. Lahoud et al., Gene targeting of desrt, a novel ARID class DNA-binding protein, causes growth retardation and abnormal development of reproductive organs, GENOME RES, 11(8), 2001, pp. 1327-1334
We have cloned and characterized a novel murine DNA-binding protein Desrt,
with a motif characteristic of the ARID (A-T rich interaction domain) famil
y of transcription factors. The Desrt gene encodes an 83-kD protein that is
shown to bind DNA and is widely expressed in adult tissues. To examine the
in vivo function of Desrt we have generated mice with a targeted mutation
in the ARID domain of Desrt. Homozygous mutants have reduced viability, pro
nounced growth retardation, and a high incidence of abnormalities of the fe
male and male reproductive organs including cryptorchidism. This may thus s
erve as a model to dissect the mechanisms involved in the development of th
e reproductive tract including testicular descent. Gene-targeted mice also
display a reduction in the thickness of the zona reticularis of the adrenal
gland and transient aberrations of the T and B cell compartments of primar
y lymphoid organs. These data show that this novel DNA-binding protein, Des
rt, has a nonredundant function during growth and in the development of the
reproductive system.