Fc. Noonan et al., Characterization of the homeodomain gene EMX2: Sequence conservation, expression analysis, and a search for mutations in endometrial cancers, GENOMICS, 76(1-3), 2001, pp. 37-44
Previous loss-of-heterozygosity studies in endometrial carcinoma mapped a p
utative tumor suppressor gene to 10q25.3-26.1. An analysis of genomic seque
nces for the deletion interval showed several expressed sequence tags and t
he homeodomain gene EMX2, a homologue of Drosophila melanogaster empty spir
acles. Expression studies showed that EMX2 transcripts are abundant in the
adult uterus and that message levels seem to be inversely correlated with e
ndometrial proliferation. EMX2 RNA was more abundant in quiescent postmenop
ausal endometrium than in premenopausal endometrium. We found decreased EM
expression in a subset of primary endometrial tumors, and four of six endom
etrial cancer cell lines investigated failed to express EMX2. The predicted
protein showed extensive amino acid conservation with EMX2 sequences from
several vertebrates. There was also considerable evolutionary conservation
in the 3 ' untranslated region. To examine the potential function of EMX2 i
n endometrial tumorigenesis, we investigated 20 primary tumors and 6 endome
trial cancer cell lines for mutations. Two primary tumors had mutations. In
activation or reduced expression of EMX2 in cancers, coupled with increased
expression in the quiescent endometrium, indicate that this homeodomain ge
ne is involved in maintenance of the differentiated state.