In vitro cleavage and development of buffalo oocytes with semen from different bulls

The present study was aimed to compare the cleavage and development rate of buffalo oocytes inseminated with semen from different bulls. Cleavage rate of zygotes reflects the potential of fertilized oocytes to divide to more than 2 cell stage. In this study frozen semen of 4 Murrah buffalo bulls was used for insemination of in vitro matured oocytes. The number of in vitro matured oocytes inseminated from 4 bulls was 122, 85, 61 and 111 respective ly. The cleavage rates were 70.4, 414, 91.8 and 72.9%, respectively, and we re significantly lower in group 2. Bulls did not affect further development of embryos to morula and blastocysts stages. The results suggested that va riation was at fertilization level and once cleavage started there was no v ariation due to bull on further development of embryos.