SUBSTITUTION OF CARBONATE BY ACETATE BUFFER FOR IGG COATING IN SANDWICH ELISA

The first step of sandwich ELISA, namely adsorption of antibodies to p lastic microtiter plates, was studied as a function of the pH of the c oating buffer. Coating efficiency was assessed in terms of maximum sig nal (absorbance) observed iu ELISA and also estimated by measuring the amount of functional antibodies adsorbed to the plate. While goat ant ibodies displayed better results after coating with acetate pH 5 buffe r, rabbit IgGs generally worked well at pH 7.4. On average, the classi cal carbonate pH 9.6 buffer was only 50% as efficient.