G. Narayanaswami et Rj. Levis, DETECTION OF OLIGONUCLEOTIDES HYBRIDIZED TO A PLANAR SURFACE USING MATRIX-ASSISTED LASER-DESORPTION MASS-SPECTROSCOPY, Journal of the American Chemical Society, 119(29), 1997, pp. 6888-6890
The detection of oligonucleotides hybridized to a planar, plastic subs
trate using matrix-assisted laser-desorption time-of-flight mass spect
roscopy is reported. Oligonucleotides of length 18 to 29 bases were hy
bridized to a plastic surface upon which a monolayer of an oligonucleo
tide of sequence 5'-TCC TCT CTC CGT CAT GCG TAT CGT TCA AT-3' was teth
ered at the 3' end. The target olignucleotides were hybridized from di
lute aqueous solution. The system was prepared for laser desorption by
using the matrix 3-hydroxypicolinic acid, deposited at 45 degrees C.
The subsequent mass spectra revealed parent molecular ion for the targ
et DNA with low-level dimerization and no discernible fragmentation.