The host plants of the native American butterfly, Pieris napi oleracea, inc
lude most wild mustards. However, garlic mustard, Alliaria petiolata, a hig
hly invasive weed that was introduced from Europe, appears to be protected
from this insect. Although adults will oviposit on the plant, most larvae o
f P. n. oleracea do not survive on garlic mustard. We used feeding bioassay
s with different larval stages of the insect to monitor the isolation and i
dentification of two bioactive constituents that could explain the natural
resistance of this plant. A novel cyanopropenyl glycoside (1), alliarinosid
e, strongly inhibits feeding by first instars, while a flavone glycoside (2
), isovitexin-6"-D-beta -glucopyranoside, deters later instars from feeding
. Interestingly, the first instars are insensitive to 2, and the late insta
rs are little affected by 1. Furthermore, differential effects of dietary e
xperience on insect responses suggest that 1 acts through a mechanism of po
st-ingestive inhibition, whereas 2 involves gustatory deterrence of feeding
.