Bonded dimethylacrylamide as a permanent coating for capillary electrophoresis

A method for coating capillaries for capillary electrophoresis with chemica lly bonded polydimethylacrylamide has been developed, and the properties of the capillaries have been evaluated. The coated capillaries provided high separation efficiency, 12.10(5) theoretical plates/m was obtained for cytoc hrome c. The electroosmotic flow at pH 8.0 was 10.10(-10) to 6.10(-10) m(2) V-1 s(-1). The coated capillaries were quite stable at high pH. At least 1 50 runs could be done at pH 10 without appreciable performance deterioratio n. The excellent performance of the coated capillaries was illustrated by s eparation of basic proteins, acidic proteins, 9-fluorenylmethyl chloroforma te-derivatized neurotransmitter amino acids, peptide reference mixtures and peptides digested from a bacteria protein. (C) 2001 Elsevier Science B.V. All fights reserved.