Capillary electrochromatographic enantioseparations using a packed capillary with a 3 mu m OD-type chiral packing

Enantiomer separations by capillary electrochromatography (CEC) using a pac ked capillary were investigated. As a chiral stationary phase, an OD-type p acking material of 3 mum particle diameter, which is a silica-gel based mat erial coated with cellulose tris(3,5-dimethylphenylcarbamate), was employed . The chiral packing was packed into a 100 mum I.D. fused-silica capillary by a pressurized method. Several racemic enantiomers were successfully reso lved with acidic or basic buffers containing acetonitrile as mobile phases. The separation efficiencies obtained in this chiral CEC system were superi or to those in the previously reported chiral CEC system using 5 mum partic les. The typical plate heights obtained for several enantiomers were 4.6-6. 6 mum or reduced plate heights of 1.5-2.2. Good repeatabilities in the migr ation time, peak height, and corrected peak area were observed as well as f or the plate number. As an application study of this CEC system, an optical purity test was carried out by using benzoin enantiomers. A 1% content of one enantiomer in the other enantiomer was successfully separated and detec ted. (C) 2001 Elsevier Science BV All rights reserved.