Capillary electrochromatography-laser-induced fluorescence method for separation and detection of dansylated dialkylamine tags in encoded combinatorial libraries

LC-fluorescence and LC-MS methods have been previously reported for use in decoding bead-based combinatorial libraries. We present the use of capillar y electrochromatography (CEC) for highly selective decoding in combination with laser-induced fluorescence (LIF) detection for high sensitivity. The r esults are compared to prior data obtained using HPLC with fluorescence det ection. The use of CEC shows promise for miniaturization and multiplexing f or future applications, and the use of LIF detection can allow for detectio n at sub-pmol amounts. (C) 2001 Elsevier Science B.V. All rights reserved.