Use of beta-cyclodextrin in the capillary zone electrophoretic separation of the components of clandestine heroin preparations

The present paper describes the methodological optimization and validation of a capillary zone electrophoresis method for the rapid determination of h eroin, secondary products and additives present in clandestine heroin sampl es, by using 20 mm beta -cyclodextrins in phosphate buffer, pH 3.23. Applie d potential was 15 kV and separation temperature was 24 degreesC; detection was by UV absorption at 200 nm wavelength. Heroin samples were first disso lved in CHCl3-MeOH (96:4, v/v) and injected by pressure (0.5 p.s.i., 3 s; 1 p.s.i. = 6894.76 Pa) after evaporation of the organic mixture and reconsti tution in aqueous buffer. Under the described conditions, phenylethylamine (internal standard), morphine, monoacetylmorphine, heroin, acetylcodeine, p apaverine, codeine and narcotine were baseline resolved in less than 10 min . The limit of detection was better than 1 mug/ml for each analyte. The stu dy of the intra-day and day-to-day precision showed, in terms of migration times, RSDs less than or equal to 0.71% and, in terms of peak areas, RSDs l ess than or equal to 3.2%. Also, the evaluation of linearity and analytical accuracy of the method provided good results for all the analytes investig ated, thus allowing its application to real cases of seized controlled drug preparations. (C) 2001 Elsevier Science BY. All rights reserved.