Determination of fluvastatin and its five metabolites in human plasma using simple gradient reversed-phase high-performance liquid chromatography with ultraviolet detection

Authors
Nakashima, A Saxer, C Niina, M Masuda, N Iwasaki, K Furukawa, K
Citation
A. Nakashima et al., Determination of fluvastatin and its five metabolites in human plasma using simple gradient reversed-phase high-performance liquid chromatography with ultraviolet detection, J CHROMAT B, 760(1), 2001, pp. 17-25
Citations number
13
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
13872273 → ACNP
Volume
760
Issue
1
Year of publication
2001
Pages
17 - 25
Database
ISI
SICI code
1387-2273(20010825)760:1<17:DOFAIF>2.0.ZU;2-4
Abstract
A simple gradient reversed-phase high-performance chromatographic method wi th ultraviolet detection for the determination of fluvastatin (FV) and its five metabolites, (M-2, M-3, M-4, M-5 and M-7) in human plasma was develope d and validated. The limit of quantification of FV and its five metabolites in human plasma was 10 ng ml(-1). The assay had satisfactory selectivity, recovery, linearity and precision accuracy. Stability studies showed that F V and its five metabolites were stable in plasma up to at least 1 month of storage at -30 degreesC. (C) 2001 Elsevier Science B.V. All rights reserved .