Simple and rapid high-performance liquid chromatography method for the determination of ofloxacin concentrations in plasma and urine

A high-performance liquid chromatographic method for the determination of o floxacin in human plasma and urine was developed. The method involved depro teinisation of the sample with perchloric acid and analysis of the supernat ant using a reversed-phase C-18 column and fluorescence detection at an exc itation wavelength of 290 nm and an emission wavelength of 460 nm. The assa y was linear from 0.5 to 10.0 mug/ml. The relative standard deviation of in tra- and inter-day assays was lower than 5%. The average recovery of ofloxa cin from plasma was 93%. The method was evaluated in samples from healthy s ubjects whose drug levels were already measured by microbiological assay. ( C) 2001 Elsevier Science B.V. All rights reserved.