Trichothecene and moniliformin production by Fusarium species from westernCanadian wheat

Fusarium graminearum, Fusarium culmorum, and Fusarium avenaceum, isolated f rom Fusarium-damaged wheat harvested in western Canada, were cultured and e valuated for mycotoxin production. Extracts of the culture media were assay ed for trichothecenes by gas chromatography-mass spectrometry and for monil iformin by liquid chromatography. Deoxynivalenol (DON) was found in 28 of 4 2 isolates of F. graminearum and 42 of 42 isolates of F. culmorum at levels ranging from 0.5 to 25.0 mug/g. 15-AcetylDON was found in 28 of 42 isolate s of F. graminearum at levels ranging from 1.0 to 7.1 mug/g. 3AcetylDON was found in 41 of 42 isolates of F. culmorum at levels ranging from 0.8 to 13 .0 mug/g. Several other trichothecenes were assayed but not detected in the culture medium. Moniliformin was present in 40 of 42 isolates of F. avenac euin at levels ranging from 1.3 to 138.1 mug/g, but was not present in any of the isolates of F. graminearum or F. culmorum.