M. Thiel et al., Effects of hypertonic saline on expression of human polymorphonuclear leukocyte adhesion molecules, J LEUK BIOL, 70(2), 2001, pp. 261-273
Hypertonic saline prevents vascular adherence of neutrophils and ameliorate
s ischemic tissue injury. We hypothesized that hypertonic saline attenuates
N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated expression of ad
hesion molecules on human polymorphonuclear leukocytes (PMNLs). fMLP-stimul
ated up-regulation of beta2-integrins was diminished by hypertonic saline b
ut not by hypertonic choline chloride-, mannitol-, or sucrose-modified Hank
s' buffered salt solution. Shedding of L-selectin was decreased by hyperton
ic saline and choline chloride but not by hypertonic mannitol or sucrose. W
hen the effects of hypertonic sodium chloride- and choline chloride-modifie
d media were compared, neither solution affected fMLP-receptor binding but
both equally inhibited fMLP-stimulated increase in intracellular calcium, i
onophore A23187, and phorbol myristate acetate (PMA)-stimulated numerical u
p-regulation of beta2-integrins. Analysis of mitogen-activated protein (MAP
) kinases p38 and p44/42 for phosphorylation revealed that hypertonic solut
ions did not differ in preventing fMLP-stimulated increases in phospho-p38
and phospho-p44/42. Resting PMNLs shrunk by hypertonic saline increased the
ir volume during incubation and further during chemotactic stimulation. Add
ition of amiloride further enhanced inhibition of up-regulation of beta2-in
tegrins. No fMLP-stimulated volume changes occurred in PMNLs exposed to hyp
ertonic choline chloride, resulting in significant cell shrinkage. Results
suggest a sodium-specific inhibitory effect on up-regulation of beta2-integ
rins of fMLP-stimulated PMNLs, which is unlikely to be caused by alteration
s of fMLP receptor binding, decrease in cytosolic calcium, attenuation of c
alcium or protein kinase C-dependent pathways, suppression of p38- or p44/4
2 MAP kinase-dependent pathways, or cellular ability to increase or decreas
e volumes.