CD36 does not play a direct role in HDL or LDL metabolism

Citation
Wjs. De Villiers et al., CD36 does not play a direct role in HDL or LDL metabolism, J LIPID RES, 42(8), 2001, pp. 1231-1238
Citations number
39
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF LIPID RESEARCH
ISSN journal
00222275 → ACNP
Volume
42
Issue
8
Year of publication
2001
Pages
1231 - 1238
Database
ISI
SICI code
0022-2275(200108)42:8<1231:CDNPAD>2.0.ZU;2-M
Abstract
CD36 and scavenger receptor class B, type I (SR-Bl) are both class B scaven ger receptors that recognize a broad variety of ligands, including oxidized low density lipoprotein (oxLDL), HDL, anionic phospholipids, and apoptotic cells. In this study we investigated the role of mouse CD36 (mCD36) as a p hysiological lipoprotein receptor. We compared the association of various l ipoprotein particles with mCD36 and mSR-BI expressed in COS cells by adenov irus-mediated gene transfer. mCD36 bound human oxLDL and mouse HDL with hig h affinity. Human LDL bound poorly to mCD36, indicating that mCD36 is unlik ely to play a significant role in LDL metabolism. The ability of mCD36 to m ediate the selective uptake of cholesteryl esters (CE) from receptor-bound HDL was assessed. In comparison with mSR-BI, mCD36 inefficiently mediated t he selective uptake of CE. Hepatic overexpression of mCD36 in C57BL/6 mice by adenovirus-mediated gene transfer did not result in significant alterati ons in plasma LDL and HDL levels.ie We conclude that mCD36, while able to b ind HDL with high affinity, does not contribute significantly to HDL or LDL metabolism.