TOWARDS A HUMAN CRYSTALLIN MAP - 2-DIMENSIONAL GEL-ELECTROPHORESIS AND COMPUTER-ANALYSIS OF WATER-SOLUBLE CRYSTALLINS FROM NORMAL AND CATARACTOUS HUMAN LENSES

This paper describes a first approach to establish a master data base of human lens crystallins obtained by computer analysis of standardize d two-dimensional lenticular protein patterns. To facilitate the event ual identification of the spots, the major crystallins have been separ ated into alpha-, beta(H)-, beta(L)- and gamma-crystallin fractions by gel filtration. The authors encourage colleague investigators to coll aborate in a common effort in order to arrive eventually at a two-dime nsional gel data base of all lenticular proteins.