Rj. Isaacs et Hp. Spielmann, NMR evidence for mechanical coupling of phosphate B-I-B-II transitions with deoxyribose conformational exchange in DNA, J MOL BIOL, 311(1), 2001, pp. 149-160
The conformational exchange of the phosphate and deoxyribose groups of the
DNA oligomers d(GCGTACGC)(2) and d(CGCTAGCG)(2) have been investigated usin
g a combination of homonuclear and heteronuclear NMR techniques. Two-state
exchange between phosphate B-I and B-II conformations and deoxyribose N and
S conformations was expressed as percent population of the major conformer
, %B-I or %S. Sequence context-dependent variations in %B-I and %S were obs
erved. The positions of the phosphate and deoxyribose equilibria provide a
quantitative measure of the ps to ns timescale dynamic exchange processes i
n the DNA backbone. Linear correlations between %B-I, %S, and previously ca
lculated model free C-13 order parameters (S-2) were observed. The %B-I of
the phosphates were found to be correlated to the S' of the flanking C3' an
d C4' atoms. The %B-I was also found to be correlated with the %S and C1' S
-2 of the deoxyribose ring 5' of the phosphates. The %B-I of opposing phosp
hates is correlated, while the %B-I of sequential phosphates is anti-correl
ated. These correlations suggest that conformational exchange processes in
DNA are coupled to each other and are modulated by DNA base sequence, which
may have important implications for DNA-protein interactions. (C) 2001 Aca
demic Press.