The mechanism of phenylephrine-mediated [Ca2+](i) oscillations underlying tonic contraction in the rabbit inferior vena cava

1. We characterized the mechanisms in vascular smooth muscle cells (VSMCs) that produce asynchronous, wave-like Ca2+ oscillations in response to pheny lephrine (PE). Confocal imaging was used to observe [Ca2+](i) in individual VSMCs of intact inferior vena cava (IVC) from rabbits. 2. It was found that the Ca2+ waves were initiated lay Ca2+ release from th e sarcoplasmic reticulum (SR) via inositol 1,4,5-trisphosphate-sensitive SR Ca2+ release channels (IP3R channels) and that refilling of the SR Ca2+ st ore through the sarcoplasmic-endoplasmic reticulum Ca2+-ATPase (SERCA) was required for maintained generation of the repetitive Ca2+ waves. 3. Blockade of L-type voltage-gated Ca2+ channels (L-type VGCCs) with nifed ipine reduced the frequency of PE-stimulated [Ca2+](i) oscillations, while additional blockade of receptor-operated channels/store-operated channels ( ROCs/SOCs) with SKF96365 abolished the remaining oscillations. Parallel for ce measurements showed that nifedipine inhibited PE-induced tonic contracti on by 27% while SKF96365 abolished it. This indicates that stimulated Ca2entry refills the SR to support the recurrent waves of SR Ca2+ release and that both L-tyke VGCCs and ROCs/SOCs contribute to this process: 4. Application of the Na+-Ca2+ exchanger (NCX) inhibitors 2',4'-dichloroben zamil (forward- and reverse-mode inhibitor) and KB-R7943 (reverse-Triode in hibitor) completely abolished the nifedipine-resistant, component of [Ca2+] (i) oscillations and markedly reduced PE-induced tone. 5. Thus, we conclude that, each Ca2+ wave depends on initial SR Ca2+ releas e via IP3R channels followed by SR Ca2+ refilling through SERCA. Na+ entry through ROCs/SOCs facilitates Ca2+ entry through the NCX operating in the r everse anode, which refills the SR and maintains PE-induced [Ca2+](i) oscil lations. In addition some Ca2+ entry through L-type VGCCs and ROCs/SOCs ser ves to modulate the frequency of the oscillations and the magnitude of forc e development.