A. Curnow et al., A preliminary investigation of the effects of arsenate on irradiation-induced DNA damage in cultured human lung fibroblasts, J TOX E H A, 63(8), 2001, pp. 605-616
Citations number
29
Categorie Soggetti
Environment/Ecology,"Pharmacology & Toxicology
Journal title
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
Single-cell gel electrophoresis ( the comet assay) was used to assess singl
e-strand breaks (SSBs) produced in cultured lung human fibroblasts by xenon
lamp irradiation alone, various concentrations of arsenate [As(V)], alone
or various combinations of the two. It was found that significantly higher
levels of SSBs were observed in the irradiated cells than the nonirradiated
cells and that elevating levels of arsenate enhanced the level of damage d
etected in both irradiated and nonirradiated cells in a concentration-depen
dent manner; that is, incubating cells with arsenate alone produced marked
DNA damage without an irradiation insult being necessary. The results of th
is study indicate that arsenate is acting as a cogenotoxin with irradiation
in this cell line. This additive effect may also be cocarcinogenic, and as
a result it is possible that less solar irradiation may be required to ind
uce skin cancer in arsenic-exposed populations.