M. Lee et al., Murine cytomegalovirus containing a mutation at open reading frame M37 is severely attenuated in growth and virulence in vivo, J VIROLOGY, 74(23), 2000, pp. 11099-11107
A pool of murine cytomegalovirus (MCMV) mutants was generated by using a Tn
3-based transposon mutagenesis procedure. One of the mutants, RvM37, which
contained the transposon sequence at open reading frame M37, was characteri
zed both in tissue culture and in immunocompetent BALB/c and immunodeficien
t SCID mice. Our results provide the first direct evidence to suggest that
M37 is not essential for viral replication in vitro in NIH 3T3 cells. Compa
red to the wild-type strain and a rescued virus that restored the M37 regio
n, the viral mutant was severely attenuated in growth in both BALB/c and SC
ID mice after intraperitoneal infection. Specifically, titers of the Smith
strain and rescued virus in the salivary glands, lungs, spleens, livers, an
d kidneys of the SCID mice at 21 days postinfection were about 5 X 10(5), 2
X 10(5), 5 X 10(4), 5 X 10(3), and 1 X 10(4) PFU/ml of organ homogenate, r
espectively; in contrast, titers of RvM37 in these organs were less than 10
(2) PFU/ml of organ homogenate. Moreover, the virulence of the mutant virus
appeared to be significantly attenuated because none of the SCID mice infe
cted with RvM37 had died by 120 days postinfection, while all animals infec
ted with the wild-type and rescued viruses had died by 26 days postinfectio
n. Our results suggest that M37 probably encodes a virulence factor and is
required for MCMV virulence in SCID mice and for optimal viral growth in vi
vo.