Comparison of viral genomic RNA sorting mechanisms in human immunodeficiency virus type 1 (HIV-1), HIV-2, and moloney murine leukemia virus

Genomic RNA sorting between translation and packaging was examined for huma n immunodeficiency virus type 1 (HIV-1) and HIV-2 using actinomycin D and l eptomycin B treatment. Both viruses behaved differently from a simple retro virus under actinomycin D treatment. With leptomycin B, the lack of apparen t functional separation between translation and packaging functions in lent iviruses was confirmed. HIV-2 RNA levels were more stable, but reverse tran scriptase production declined similarly to HIV-1.