In vivo detection of C-13-enriched glucose metabolites in mouse brain by T-SEDOR imaging

Protons J-coupled to C-13 were selectively detected in the mouse head by in vivo H-1 NMR imaging based on Twin Spin Echo DOuble Resonance (T-SEDOR) ex citation. This pulse sequence combines a good chemical specificity with hig h sensitivity, requires no solvent pre-saturation and is well adapted to th e imaging modality. H-1 T-SEDOR maps of the mouse head allowed detection of areas of preferential accumulation of C-13-enriched compounds, upon repeat ed injections of uniformly C-13-labelled glucose, which induced hyperglycem ia. The results demonstrated the feasibility, both in time scale and metabo lite concentration, of applying T-SEDOR MRI for in vivo mapping brain areas characterized by enhanced rates of glucose uptake and/or accumulation of i ts metabolites. (C) 2001 Elsevier Science Inc. All rights reserved.