DNA microarrays and beyond: completing the journey from tissue to cell

For the cell biologist, identifying changes in gene expression using DNA mi croarrays is,just the start of along journey from tissue to cell. We discus s how chip users can first filter noise (false-positives) from daunting mic roarray datasets. Combining laser capture microdissection with real-time po lymerase chain reaction and reverse transcription is a helpful follow-up st ep that allows expression of selected genes to be quantified in populations of recovered cells. The voyage from chip to single cell can be completed u sing sensitive new in situ hybridization and immunohistochemical methods ba sed on tyramide signal amplification.