MRNA expression of the IGF system in the kidney of the hypersomatotropic rat

Aim: To examine the response of the insulinlike growth factor (IGF) system in the kidney during a state of extreme growth. Methods: We studied the mRN A expression of IGF-I, IGF-I receptor, and IGF-binding proteins (BP) using sensitive RNase protection assays following subcutaneous implantation of gr owth hormone pituitary cells (GH(3)) in rats. Results: Within 5 weeks, the serum GH levels increased from 18.0 +/- (SE) 5.0 ng/ml in control animals t o 389.8 +/- 30.3 ng/ml in GH3 rats (n = 5, p < 0.001). The circulating IGF- l levels were also elevated. The kidney weights increased from 0.74 +/- 0.0 1 g in controls to 1.06 +/- 0.03 g in GH3 animals (n 5, p < 0.001). Similar changes were observed at week 10. The renal IGF-l mRNA averaged 1.0 +/- (S D) 0.33 relative densitometry units in controls (n = 4) and increased to 2. 11 +/- 0.13 relative densitometry units in GH3 rats (n = 5, p < 0.001). On the other hand, mRNA for the type I IGF receptor decreased in hypersomatotr opic rats. Messenger RNAs for IGFBP-1 and IGFBP-4, which have been localize d to renal tubules, both decreased significantly following growth induction , while IGFBP-3, the mRNA of which has an interstitial localization, was in creased at week 10. Conclusion: These data suggest that there is a dynamic relationship between tubular and interstitial compartments with regard to t he IGF system in the kidney which may be important in the regulation of the cell mass. Copyright (C) 2001 S. Karger AG, Basel.