J. Hovinen et H. Hakala, Versatile strategy for oligonucleotide derivatization. Introduction of lanthanide(III) chelates to oligonucleotides, ORG LETT, 3(16), 2001, pp. 2473-2476
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Novel nucleosidic phosphoramidite blocks were synthesized by a Mitsunobu re
action between 2'-deoxy-5'-O-(4,4'-dimethoxytrityl)uridine and a primary al
cohol containing a conjugate group in its structure (a protected functional
group, an organic dye, or a precursor of a lanthanide(III) chelate) follow
ed by phosphitylation. They were used in machine-assisted DNA synthesis in
the standard manner. A slighty modified deprotection procedure was used for
the preparation of oligonucleotide conjugates tethered to lanthanide(Ill)
chelates. For the latter application one non-nucleosidic block was also syn
thesized.