Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana

Pyk10 is a root and hypocotyl specific myrosinase from Arabidopsis thaliana . Northern analysis revealed the root specific expression of pyk10. In orde r to study the pyk10 promoter and the genomic structure of the gene, a geno mic clone was isolated and sequenced. The clone contained the complete pyk1 0 gene and a promoter region of 3569 bp. The gene spans 2963 by and consist s of 12 exons and 11 introns, a structure that reflects the common gene org anization of myrosinases. Within the promoter sequence, different developme nt specific, organ specific, elicitor and plant hormone responsive regulato ry elements could be identified, which also occur in other promoters. To de termine the pattern of expression, four different 5 ' -promoter deletion fr agments were linked to a beta -glucuronidase (gus) reporter gene and transf ormed into A. thaliana. The results demonstrated that the pyk10 promoter me diates a developmental gene activity with a strong emphasis in the root. Ci s-acting sequences regulating root specific expression were identified to r eside in the two promoter fragments B and C. (C) 2001 Elsevier Science Irel and Ltd. All rights reserved.