J. Seghatchian et al., Studies on the improvement of leucodepletion performance of the Haemonetics MCS plus for production of leucodepleted platelet concentrate, PLATELETS, 12(5), 2001, pp. 298-301
With the implementation of universal leucodepletion, an in-line, negatively
charged LRF6H leucodepleting filter became an essential part of the Haemon
etics MCS+ plateletpheresis system. A larger-scale (968) study using the st
andard protocol revealed a 2.79% leucodepletion failure rate (standard < 5
x 10(6) leucocytes per adult therapeutic dose). Factors influencing the eff
icacy of the filter were investigated. The pH of the filtrate was 7.0, the
temperature 28<degrees>C and filtration rate 80 ml/min. Reduction of the fi
ltration rate to 30 ml/min (784 doses) reduced leucodepletion failure to 0.
38%. Measurement of the leucocyte count, pre- and post-filtration of the pl
atelet products, revealed that donations from 1% of donors contained substa
ntially larger numbers of leucocytes in pre-filter samples (300-1500/(mu)l)
than in control samples (35-70/(mu)l). This number tends to increase progr
essively with subsequent donations in these individuals, leading to leucode
pletion failure, whilst peripheral leucocyte counts remain normal. The new
continuous filtration protocol (version C) using a less impact filter LRF-X
L and a lower (7 ml/min) head pressure was also effective but failure still
occurred twice on one of the donors who persistently showed high pre-filte
r count. We conclude that leucodepletion failures in the Haemonetics system
are related to both donor leucocyte (i.e., being light and non-adherent) a
nd operational/filter performance.